He distance from the center of the anterior border of the reattached muscle to PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20460822 the point just anterior to the original insertion site was grossly measured with calipers (Figures 2c, d). The anterior border of the new insertion was defined as the point just anterior to the engaged portion of the muscle by muscle hook. Anterior displacement was defined the final insertion was 0.5 mm or more anterior than the intended insertion and posterior displacement was the final insertion was 0.5 mm or more posterior than the intended insertion. Four eyes from two randomly selected rabbits were enucleated and fixed in 10 neutral buffered formalin. Globes were dissected in the sagittal plane and routinely embedded in paraffin. Four-micrometer thick sectionswere stained with hematoxylin and eosin and studied under the light microscope. We confirmed that the displacement of recessed muscle in 32 eyes of 16 rabbits followed a normal distribution using Kolmogorov-Smirnov test. The Fisher's exact test and the Pearson chi-square test was applied to compare the stability of recessed muscle between the two groups--hang-back recession alone (control group) and hang-back recession in combination with fibrin glue (fibrin glue group) and t-test was used to compare the absolute value of the displacement. SPSS (version 15.0) was used for statistical analysis, with statistical significance defined as a p value less than 0.05.Results The average distances from the original insertion site to the postoperative insertion site just after surgery were 3.94 ?0.17 mm in the control group and 3.97 ?0.22 mm in the fibrin glue group (p = 0.670) (Table 1). Six weeks after the surgery, some superior rectus PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/3081428 muscles were displaced forward from the intended site, and some were displaced backward, in both groups. In the control group, five eyes (31.3 ) showed anterior displacement (0.60 ?0.22 mm, range 0.50 1.00 mm), and eight eyes (50.0 ) showed posterior displacement (0.69 ?0.26 mm, range 0.50 1.00 mm). In the fibrin glue group, one eye (6.3 ) showed anterior displacement (0.5 mm), and six eyes (37.5 ) showed posterior displacement (0.92 ?0.38 mm, range 0.50 1.50 mm). The superior rectus muscle was attached to the intended site in three eyes in the control group (18.8 ) and in nine eyes in the fibrin glue group (56.3 ) (p = 0.028) (Table 2). The control group and the fibrin glue group showed similar histological findings on microscopic examination. The sclera consisted of fine-textured collagen fibrous bundles, which were parallel to the surface of the globe. The collagen fibers in the left eye were partially cut off at the muscle insertion site, but they were nearly intact as a whole. The rectus muscle at the insertion tert-Butyl (7-bromoheptyl)carbamate site was separated from the scleral surface and surrounded by fibrous tissue, which was attached to the sclera. Residual fibrin glue or inflammatory cells were rarely seen (Figure 3b). The eye in the control group was not significantly different from the eye in the fibrin glue group, but it was characterized by more red blood cells (Figure 3a). The surrounding connective tissue didn't show any significant inflammatory change. Discussion 3-Nitro-6-(trifluoromethyl)pyridin-2(1H)-one There are chances of scleral perforation in the high risk situations such as thin sclera and restricted field of the operation, and in those cases, we can choose the modified suspension-recession technique or hang-back recession [20]. Several reports on hang-back recession inFigure 1 Fibrin glue (Greenplast kitW). Human fibri.